Purification of human sperm by a discontinuous Percoll density gradient with an innercolumn
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چکیده
منابع مشابه
Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient.
Cryptosporidium oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by density gradient centrifugation. This two-step method yielded highly concentrated oocysts largely free of bacteria and fecal debris.
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Silane-coated silica particle solutions (ISolate(TM) and PureSperm)TM)) and iodixanol (OptiPrep(TM)) were compared to polyvinylpyrrolidone (PVP)-coated silica particles (Percoll(TM)) in their efficacy to recover spermatozoa by gradient centrifugation for use in assisted reproductive procedures. Efficacy was assessed in terms of percentages of sperm recovery, sperm vitality and motility, normal ...
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Synaptoneurosomes (SNs) are obtained after homogenization and fractionation of mouse brain cortex. They are resealed vesicles or isolated terminals that break away from axon terminals when the cortical tissue is homogenized. The SNs retain pre- and postsynaptic characteristics, which makes them useful in the study of synaptic transmission. They retain the molecular machinery used in neuronal si...
متن کاملComparison of the coverslip and the discontinuous Percoll density gradient methods of enucleation of mouse cells.
Two methods of enucleation of LB 10 cells, a subline of mouse L cells, were used: the method of enucleation of cells growing in monolayers, and the newly improved method of enucleation in discontinuous Percoll gradients. The second method was more effective and, as shown by incorporation of 3H-lysine, protein synthesis in cytoplasts was prolonged twice when compared with that in cytoplasts obta...
متن کاملBull Fertility and Its Relation with Density Gradient Selected Sperm
Background sperm selection is generally used to describe methods for separation of spermatozoa for in-vitro-assisted reproduction. Few studies reported relation in vivo fertility and semen parameters after selection of fertile spermatozoa; hence, our research attempt to assess different semen parameters after post-thaw (PT) or after selecting sperm sample (SSp), using density gradient separatio...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1986
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod35.4.1059